![]() ![]() Size-exclusion chromatography was applied to the isolation of EVs from whole T. circumcincta EVs, and whole and EV-depleted ESPs, using bacterial growth inhibition assays. In the current study, we (i) undertake proteomic analyses of the excretory-secretory products (ESPs), including secreted extracellular vesicles (EVs), of the ‘brown stomach worm’ Teladorsagia circumcincta, one of the major agents causing parasite gastroenteritis in temperate areas worldwide (ii) conduct bioinformatic analyses to identify and characterise antimicrobial peptides (AMPs) with putative antimicrobial activity and (iii) assess the bactericidal and/or bacteriostatic properties of T. The trinket might be a reference to Parelaphostrongylus tenuis, a real life brain worm commonly found in white-tailed deer.Over the past decade, evidence has emerged of the ability of gastrointestinal (GI) helminth parasites to alter the composition of the host gut microbiome however, the mechanism(s) underpinning such interactions remain unclear.The name, appearance and effect of the trinket are likely inspired by the Brain enemy.Brain Worm originates from the Antibirth mod.Eye of the Occult: Tears briefly snap to target enemies but Isaac's control quickly takes over them.Cricket's Body / The Parasite: Both the main and split tears are affected by Brain Worm.Brimstone / Montezuma's Revenge / Revelation / Technology / Technology 2: Laser will snap regardless of length from enemy.Anti-Gravity: Tears will snap to enemies after they're released, but not while the tears are staying in place.The effect will not work if Isaac is too close to enemies. ![]() Tears snap 90 degrees to target enemies that they may have missed. ![]()
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